ATM Knockout cell line (22Rv1)

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ATM Knockout cell line (22Rv1) Order

ATM Knockout cell line (22Rv1)
Catalog#
YKO-H222
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ATM Knockout cell line (22Rv1)
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Detailed Product Information

Product category
KO Cell Lines
cell name
ATM Knockout cell line（22RV1）
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:3
Species
Human
Gene Name
ATM
Gene ID
472
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90% RPMI-1640+10% FBS
Accession
CVCL_D7FW(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 3 (based on transcript ATM-229)
Validation: PCR+Sanger Sequencing
ATM Knockout cell line (22Rv1) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:22RV1		 STR Info (Cell bank)
Cell Line:22RV1
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 1113111213.0
D13S317 912912
D7S820 91011.010119.0
D16S539 12121212
VWA 15211521
TH01 69.369.3
AMEL XYXY
TPOX 8888
CSF1PO 10111011
D12S391 1825
FGA 2023
D2S1338 1718
D21S11 2930
D18S51 1314
D8S1179 1314
D3S1358 1415
D6S1043 1616
PENTAE 51314.0
D19S433 1314
PENTAD 912
D1S1656 171819.0
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ATM gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the 22RV1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ATM gene knockout ATM Knockout cell line（22RV1） will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
ATM Knockout cell line (22Rv1)
ATM Knockout cell line (22Rv1)
Catalog#
YKO-H222
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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